实验室安全是研究者的身体健康以及教学和科研活动顺利开展的重要保障。本书采用全英文编写，以生命科学基础实验、综合性自主实验、开放性研究实验以及科学研究活动为对象，以用于基础教学的一级生物安全水平基础实验室为参照，针对实验过程中易发生的安全事故隐患及防范措施进行分类陈述，图文并茂，通俗易懂。 本书可作为高等院校相关专业留学生或双语教学的推荐教材，也可供开展实验室工作的科研和管理人员参考。
★本书内容简明实用，仪器安全操作规范除了文字叙述外，还以操作流程图表示；安全注意事项以绘图的形式重点展示。通过图文并茂和虚实互补，突出重点和细节，有很强的针对性和可操作性，通俗易懂。
★本书每章节后配套检测习题，使实验学习更方便！
This procedure guideline was developed by Experimental Teaching Center for Life Science and Technology of Huazhong University of Science and Technology, College of Life Science and Technology to provide sufficient information on safety protection of workers and the surrounding environment in bioscience laboratory. This guideline is expected to be revised up to date guaranteeing that regulations, policies and procedures follow applicable and safe standards. All users in bioscience laboratories of educational institutions, including researchers, teachers, and students, are required to study and implement safety precautions to avoid safety accidents. This book aims to protect operators, experiment equipment and the research environment to reduce or eliminate the potential of biohazards. Neither any harms to the health of the operators nor any intentional failure of equipment is expected during bioscience experiments. Also, the research environment shall be kept fine for continuous work. Especially targeted to be applied to laboratories in general bio-laboratories for undergraduate and postgraduate education, this manual illustrates detailed procedure and guidelines for laboratory water, electricity, gas, sound, light usage and for some common apparatus. The contents are formulated on fundamental laboratory safety knowledge, standard equipment operations, the main properties of chemicals, and past laboratory experiences. The contents are concise and practical. In addition to text description, there are also standard operation procedure figures in standard operations of equipment part. Safety precautions are illustrated with figures. Therefore, this guideline is excellent with pictures and texts and are easy to understand. In addition, this guideline is scripted mainly based on Laboratory Biosafety Manual (3rd Edition, WHO), General requirements for laboratory biosafety(GB19489-2008), the Control of Substances Hazard to Health (COSHH) (http://www.hse.gov.uk/), the Globally Harmonized System of Classification and Labeling of Chemicals (GHS) etc. Moreover, this book is edited by first-line researchers in bioscience laboratories who have rich research and teaching experience, so the contents is specific and has practice guiding significance for users of bioscience laboratories.
Content
Chapter 1 Introduction to Basic Safety in
Bio-Laboratory 4
1.1 Introduction to biosafety levels and
general precautions in bioscience laboratory 4
1.2 Safety guidelines on water usage in
bioscience laboratory 6
1.3 Safety guidelines on electricity usage
in bioscience laboratory 8
1.4 Safety guidelines on gas usage in
bioscience laboratory 10
1.5 Safety guidelines on sound usage in
bioscience laboratory 12
1.6 Safety guidelines on light usage in
bioscience laboratory 13
Chapter 2 Standard Operations and Safety
Precautions of General Apparatus in Bio-Laboratory 16
2.1 Standard operations and safety
precautions of autoclave
usage.......................................................................................................16
2.2 Standard operations and safety
precautions of oven usage 20
2.3 Standard operations and safety
precautions of ultra-low temperature freezer 22
2.4 Standard operations and safety
precautions of liquid nitrogen container 23
2.5 Standard operations and safety
precautions of centrifuge .......................................................................................................24
2.6 Standard operations and safety
precautions of biosafety cabinet 28
2.7 Standard operations and safety
precautions of clean bench 30
2.8 Standard operations and safety precautions
of fume hood
........................................................................................................32
2.9 Standard operations and safety
precautions of UV analyzer 33
2.10 Standard operations and safety
precautions of laser 35
2.11 Standard operations and safety
precautions of laser power meter 37
2.12 Standard operation and safety
precaution of biological signal acquisition and analysis system 39
Chapter 3 General Safety Guidelines for
Common Biochemical Reagents Usages 42
3.1 Toxic reagents 42
3.2 Chemical unstable reagents 49
3.3 Animal anesthetics 53
Chapter 4 Storage and Handling Guidelines
of Experimental Biomaterials 55
4.1 Storage and handling guideline of
biomaterials in the life science laboratory 55
4.2 Blood samples of human and animal 61
4.3 Microorganism 63
4.4 Biomacromolecule 64
Chapter 5 Bio-science Safety, Emergency
Care and First Aid 66
5.1 Bio-laboratory potential fire accidents
66
5.2 Explosive Accidents 69
5.3 Poisoning and burn accidents 71
5.4 First aid medical box in bio-laboratory
75
References 76
新书资讯
